General information
L-glycine is the simplest, but essential amino acid in all living organisms and plays an important role as building block, metabolic pathway intermediate, signalling molecule, source for enzyme cofactor synthesis or as a supplement for cell growth. Cupriavidus necator H16 (Ralstonia eutropha) exhibits a broad spectrum and is known for its capacity to synthesizes biocatalysts,
especially O2-tolerant [NiFe}-Hydrogenases (Hyds). By harnessing the full biopotential of this biotechnologically important bacterium by optimizing the heterotrophic growth conditions, it is
possible to produce biofuels and bioproducts on a large scale. Recently performed experiments identified that some L-Amino acids, such as Glycine and Proline used in 0.7 mmol/ml concentration significantly improve growth and, in addition, enhance and prolong the synthesis of O2-tolerant Hyds (Iskandaryan et al., 2023). Therefore, it is crucial to study the optimized conditions when utilizing L-glycin is possible to achieve enhanced biomass containing active hydrogenases of C. necator H16. Additionally, it is imperative to isolate and purify these active enzymes and perform biochemical analyses.
