General information
The neuromediator dopamine is an important biologically active compound in CNS. The main centers of dopamine formation are localized in regions of the middle brain. Currently, many neurophysiological and psychological problems are widespread and result from the concentration changes of this neuromediator. Consequently, the resolution of the problem that will allow to determine physiological amounts of dopamine in human organism at both normal and pathological states is sharply actual. Such diagnosis can be implemented throughout biosensor technologies. The presented project proposes observing synthetic single-stranded and doublestranded polyribonucleotides - poly(rA), poly(rU), poly(rG), poly(rC) and poly(rA)-poly(rU), poly(rG)-poly(rC) as the biosensor basis for dopamine. It is planned to reveal the interaction peculiarities of dopamine with the polynucleotides mentioned above. Besides, in the dopaminepolynucleotide system, the role of acridine orange and methylene blue is planned to examine as modulators of the obtaining signal. The polynucleotide sequences, as mentioned earlier, are prominent regulators during m-RNA processing and translation regulation in various states. Hence, dopamine interaction with them directly will show its participation in gene expression.
