General information
During biotechnological production processes, different carbon sources or their mixtures are used. The concentration and type of substrate can greatly influence the production of the desired fermentation products. This study aims to elucidate the role of global transcriptional regulators—CRP, FNR, and ArcAB—in glucose metabolism and how different glucose concentrations impact Escherichia coli's cell physiology, bioenergetics, and metabolic parameters. Understanding these regulatory mechanisms is crucial for optimizing biotechnological processes. Despite extensive research on bacterial global regulator systems, gaps remain in how these systems function under different environmental conditions during anaerobic fermentation. By studying E. coli parental strains and mutants lacking specific global regulators, the project seeks to uncover adaptive mechanisms to environmental changes. This knowledge will enhance microbial physiology understanding, improve microbial growth conditions, and refine industrial cultivation processes. The research will be conducted under Professor Gary Sawers' mentorship at Martin[1]Luther University, Germany, whose expertise and collaborative history with the applicant' supervisor ensure the project's success.
