Laboratory of Biophysics of Subcellular Structures

The Laboratory of Biophysics of Subcellular Structures was founded in 2005.

The field of laboratory research includes:

• study of nucleic acids-ligand interaction (study of biologically active compounds binding to double-stranded DNA, and single-stranded RNA by different mechanisms),

• biologically active, including the interaction of pharmacological compounds with single-stranded DNA, as well as synthetic deoxyhomopolynucleotides,

• interactions of DNA-specific compounds with blood serum protein albumin,

• influence of millimeter-range electromagnetic waves on biological systems at different levels of formation.

The main scientific directions operating in the laboratory

• Studies of the interaction of biologically active compounds with biomacromolecules, DNA, RNA, and proteins using absorption spectroscopy methods,

• The study of nucleic acids, proteins, and their complexes with biologically active compounds using the UM-denaturation method,

• Analysis of the binding of biologically active compounds to biomacromolecules by the Scatchard plot,

• Study of fluorescence properties of complexes of biologically active compounds with biomacromolecules.

Partnerships

The laboratory cooperates with the following institutions:

• RA NAS Laboratory of "Toxicological Research" of the Institute of Physiology named after L.A. Orbeli

• RA NAS "Liquid Phase Free-Radical Reactions" Laboratory of the A. Nalbandian Institute of Chemical Physics,

• Department of Molecular, Cellular and Developmental Biology, University of Michigan (USA),

• Chair of Molecular Physics of YSU Institute of Physics,

• Department of Physical and Colloid Chemistry of YSU Chemistry Faculty,

• Department of Medical Biochemistry and Biotechnology of the Armenian-Russian (Slavic) University.

Staff

1. Poghos Vardevanyan
2. Marine Parsadanyan
3. Mariam Shahinyan
4. Gayane Ananyan
5. Arsen Karapetyan
6. Aram Aramyan
7. Anna Nikolyan

Top 5 publications (Scopus, Web of Science 2019-2024)

1. Vardevanyan P.O., Parsadanyan M.A., Antonyan A.P., Shahinyan M.A., Karapetyan A.T. Spectroscopic study of interaction of various GCcontent DNA with Hoechst 33258 depending on Na + concentration. J. of Biomol. Struct. and Dyn., 2021, v. 39, N4, p. 1519-152. 
2. Vardevanyan P.O., Parsadanyan M.A., Antonyan A.P., Shahinyan M.A. Study of complexes of Hoechst 33258 with poly(rA)-poly(rU) depending on various ionic strengths in the water-saline solution. J. of Biomol. Struct. and Dyn., 2022, V.40, N3, p. 1182-1188. 
3. Vardevanyan P.O., Antonyan A.P., Movsisyan Z.H., Parsadanyan M.A., Shahinyan M.A., Grigoryan K.R., Shilajyan H.A. Study of complexation of single-stranded poly(rA) and poly(rU) with methylene blue. J. of Biomol. Struct and Dyn., 2023, V.41, N24, p. 15320-15327
4. Vardevanyan P.O., Antonyan A.P., Matevosyan A.G., Parsadanyan M.A., Movsisyan Z.O. Affinity degree assessment of DNA-specific ligands- intercalators Ehidium bromide and Methylene blue with poly(rA), poly(rU) and poly(rA)-poly(rU). Journal of Applied Spectroscopy, 2024, V. 91, N 1, p. 65-73.
5. Parsadanyan M.A., Shahinyan M.A., Mikaelyan M.S., Grigoryan S.V., Poghosyan G.H., Vardevanyan P.O. Influence of millimeter range electromagnetic waves on bovine serum albumin interaction with acridine orange. Electromagn. Biol. Med., 2024, v. 43, N4, p. 246-255.