General information
To increase bioethanol production yield, it is important to enhance yeast adaptability to the various stress factors, including acetic acid stress.
This project aims to investigate the molecular mechanisms underlying the response of industrial Saccharomyces cerevisiae yeast strains to acetic acid stress. The study will explore growth parameters, reactive oxygen species (ROS) levels, and antioxidant capacity changes in yeast under acetic acid stress. The research project will incorporate fluorescence microscopy and flow cytometry to examine intracellular ROS levels utilizing the probe 2,7-dichlorodihydrofluorescein diacetate (H2DCFDA), providing crucial insights into the oxidative stress response mechanisms of these yeast strains under varying pH conditions during respiratory or fermentative metabolism.
The project anticipates valuable insights into yeast adaptation to stress conditions, contributing to biotechnological advancements in bioethanol production and fostering international collaboration.
