Royal jelly as a natural remedy for antistress – metabolic changes and identification of stress proteins in yeasts

The development of strategies to safeguard living organisms from harmful agents has become critical. To this end, the use of royal jelly (RJ) as a natural intervention against stress is recommended. This project aims to study the molecular mechanisms of influence of RJ on yeast cells. This project aims to conduct a comprehensive investigation into the concurrent alterations in the activities of key enzymes involved in nucleotide exchange, glycolysis, the tricarboxylic acid cycle, and ornithine cycle in yeasts exposed to UV irradiation, microwaves, and heat shock. In this study, it is planned also to elucidate the interrelationships among these changes and evaluate the impact of RJ on these processes. Additionally, the project will involve conducting electrophoretic analysis of water-soluble proteins in yeasts cultured with and without RJ, assessing alterations in sub-fraction composition, and employing mass spectrometry for the identification of these proteins. As an international collaborator on this project, we intend to invite Dr Stéphane Guyot, PhD, a lecturer at the Agricultural University of Dijon (France). We plan to collaborate with the Armenian Association of Mass Spectrometry to conduct mass spectrometric studies of the proteins.